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anti icam1 primary antibody  (R&D Systems)


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    R&D Systems anti icam1 primary antibody
    <t>ICAM1</t> mRNA expression in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells (A) and HUVECs (B) were incubated for 24 h with macrophage-conditioned media. mRNA expression of ICAM1 in EA.hy926 cells (A) and HUVECs (B) was assessed by RT-qPCR (n = 4, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with EGM-2 medium. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.
    Anti Icam1 Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti icam1 primary antibody/product/R&D Systems
    Average 95 stars, based on 246 article reviews
    anti icam1 primary antibody - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasis"

    Article Title: The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasis

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2022.961753

    ICAM1 mRNA expression in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells (A) and HUVECs (B) were incubated for 24 h with macrophage-conditioned media. mRNA expression of ICAM1 in EA.hy926 cells (A) and HUVECs (B) was assessed by RT-qPCR (n = 4, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with EGM-2 medium. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.
    Figure Legend Snippet: ICAM1 mRNA expression in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells (A) and HUVECs (B) were incubated for 24 h with macrophage-conditioned media. mRNA expression of ICAM1 in EA.hy926 cells (A) and HUVECs (B) was assessed by RT-qPCR (n = 4, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with EGM-2 medium. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.

    Techniques Used: Expressing, Incubation, Derivative Assay, Quantitative RT-PCR

    ICAM1 protein abundance in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells and HUVECs were incubated for 48 h with macrophage-conditioned media. ICAM1 protein abundance in EA.hy926 cells (A) and HUVECs (B) was analyzed by immunofluorescence (n = 2). ICAM1 protein abundance in EA.hy926 cells (C) was analyzed by western blot (n = 3, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with DHGL-1 (A) or EGM-2 (B) . Incubation of endothelial cells with TNFα (1 ng/ml) for 16 h was used as positive control. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.
    Figure Legend Snippet: ICAM1 protein abundance in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells and HUVECs were incubated for 48 h with macrophage-conditioned media. ICAM1 protein abundance in EA.hy926 cells (A) and HUVECs (B) was analyzed by immunofluorescence (n = 2). ICAM1 protein abundance in EA.hy926 cells (C) was analyzed by western blot (n = 3, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with DHGL-1 (A) or EGM-2 (B) . Incubation of endothelial cells with TNFα (1 ng/ml) for 16 h was used as positive control. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.

    Techniques Used: Quantitative Proteomics, Incubation, Derivative Assay, Immunofluorescence, Western Blot, Positive Control



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    Image Search Results


    ICAM1 mRNA expression in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells (A) and HUVECs (B) were incubated for 24 h with macrophage-conditioned media. mRNA expression of ICAM1 in EA.hy926 cells (A) and HUVECs (B) was assessed by RT-qPCR (n = 4, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with EGM-2 medium. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.

    Journal: Frontiers in Oncology

    Article Title: The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasis

    doi: 10.3389/fonc.2022.961753

    Figure Lengend Snippet: ICAM1 mRNA expression in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells (A) and HUVECs (B) were incubated for 24 h with macrophage-conditioned media. mRNA expression of ICAM1 in EA.hy926 cells (A) and HUVECs (B) was assessed by RT-qPCR (n = 4, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with EGM-2 medium. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.

    Article Snippet: Cells were washed three times in PBS and were blocked in PBS containing 2% of bovine serum albumin (BSA) for 30 min. Then, cells were incubated overnight (O/N) at 4°C with anti-ICAM1 primary antibody (diluted 1:60 in 2% BSA in PBS; #BBA3, R&D Systems).

    Techniques: Expressing, Incubation, Derivative Assay, Quantitative RT-PCR

    ICAM1 protein abundance in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells and HUVECs were incubated for 48 h with macrophage-conditioned media. ICAM1 protein abundance in EA.hy926 cells (A) and HUVECs (B) was analyzed by immunofluorescence (n = 2). ICAM1 protein abundance in EA.hy926 cells (C) was analyzed by western blot (n = 3, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with DHGL-1 (A) or EGM-2 (B) . Incubation of endothelial cells with TNFα (1 ng/ml) for 16 h was used as positive control. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.

    Journal: Frontiers in Oncology

    Article Title: The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasis

    doi: 10.3389/fonc.2022.961753

    Figure Lengend Snippet: ICAM1 protein abundance in EA.hy926 cells and HUVECs incubated with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells and HUVECs were incubated for 48 h with macrophage-conditioned media. ICAM1 protein abundance in EA.hy926 cells (A) and HUVECs (B) was analyzed by immunofluorescence (n = 2). ICAM1 protein abundance in EA.hy926 cells (C) was analyzed by western blot (n = 3, mean ± 1 SEM). Ctrl 1 corresponds to endothelial cells incubated with CO 2 -independent medium. Ctrl 2 corresponds to endothelial cells incubated with DHGL-1 (A) or EGM-2 (B) . Incubation of endothelial cells with TNFα (1 ng/ml) for 16 h was used as positive control. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.

    Article Snippet: Cells were washed three times in PBS and were blocked in PBS containing 2% of bovine serum albumin (BSA) for 30 min. Then, cells were incubated overnight (O/N) at 4°C with anti-ICAM1 primary antibody (diluted 1:60 in 2% BSA in PBS; #BBA3, R&D Systems).

    Techniques: Quantitative Proteomics, Incubation, Derivative Assay, Immunofluorescence, Western Blot, Positive Control